FIGURE 5.

HCMV IE86 protein reduces the level of STING protein. (A) HFF cells were either mock-infected or infected with HCMV-Towne. At 24 h after infection, cells were harvested, and equal amounts of cell extracts were subjected to western blot analysis with antibodies to STING, HCMV IE and tubulin. (B,C) HFF cells were transduced with 10 pfu per cell of either Ad-GFP plus Ad-Trans, Ad-IE72 plus Ad-Trans or Ad-IE86 plus Ad-Trans and incubated for 48 h. (B) Cells were harvested, and equal amounts of cell extracts were subjected to western blot analysis with antibodies to STING, TBK1, HCMV IE and tubulin. (C) The level of STING mRNA was analyzed by qRT-PCR analysis. Real-time PCR data shown here represent three independent experiments ±SD.