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. 2017 Sep 26;8:1854. doi: 10.3389/fmicb.2017.01854

FIGURE 6.

FIGURE 6

HCMV IE86 protein induces the proteasome-dependent degradation of STING. HFF cells were transduced with 10 pfu per cell of either Ad-GFP plus Ad-Trans or Ad-IE86 plus Ad-Trans and incubated for 48 h. (A) Cells were treated with DMSO (vehicle for MG132 and epoxomicin, lanes 1 and 4), MG132 (lanes 2 and 5), epoxomicin (lanes 3 and 6), ddH2O (vehicle for chloroquine, lanes 7 and 9) or chloroquine (lanes 8 and 10). At 12 h after treatment, cells were harvested, and equal amounts of cell extracts were subjected to western blot analysis with antibodies to STING, HCMV IE86, LC3B and tubulin. (B) Cells were pre-treated with MG132 for 12 h and followed by a cycloheximide chase for the indicated time points. Equal amounts of cell extracts were subjected to western blot analysis with antibodies to STING, TBK1, HCMV IE86 and tubulin. The signal intensity of protein bands was analyzed using Image LabTM software for determining relative protein levels of STING at the indicated chase time points. Epox, epoxomicin; CQ, chloroquine; DW, ddH2O; CHX, cycloheximide.