Figure 5.

FLCN mediates EGF-induced Rab35 activation. (A) HeLa cells were transiently transfected with negative control siRNA or FLCN siRNA pool. At 36 h after transfection, cells were serum-starved overnight and stimulated with 10 ng/ml EGF for 15 min. Cells were lysed and subjected to GST-pulldown analysis for the activity of Rab35. ^**P < 0.01 show the comparison between groups with EGF stimulation and without EGF stimulation, ns (not significant). (B) HeLa cells were serum-free overnight and then treated with 10 ng/ml EGF for 15 min. Cells were lysed and anti-Rab35 or IgG immunoprecipitates were analyzed for coprecipitation of FLCN by western blot. Unprocessed lysates of HeLa cells was used as input.