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EGFR degradation requires Rab35 activation. (A) HeLa cells were transiently transfected with Rab35 WT or Rab35 DN plasmid and pCMV-Flag empty vector or Flag-FLCN at the same time. Cells were lysed and subjected to western blot analysis for EGFR expression. (B) FLCN-silenced HeLa cells were transiently transfected with Rab35 CA or Rab35 DN plasmid. Cells were lysed and subjected to western blot analysis for EGFR expression.
