Figure 8.

Cdh13 deficiency affects the cell density and area of the dorsal raphe, but not the median raphe, at E13.5. (A) Representative images of DR stained with 5-HT are shown. A significantly higher number of serotonergic neurons (P = 0.0014) within a predetermined area (397.5 μm²) and a trend toward reduced DR area (P = 0.051) is observed in Cdh13^−/− mice compared to wildtype controls. n = 8 per genotype. (B) Representative images of MR stained with 5-HT are shown. No significant differences in the density of serotonergic neurons in the MR between Cdh13^−/− and wildtype embryos is observed. n = 4 per genotype. Orientation: sagittal. Scale bar in (A,B): 100 μm. Data are presented as mean ± s.e.m. ^#P < 0.1, ^**P < 0.01.