Table 2.
PCR primer sequences using for MeDIP
| Target genes | MeDIP primer sequence | GenBank accession No. | PCR products, bp |
|---|---|---|---|
| FADS2 | F:5′-GGCACTAATCCCAAGCAG-3’ | XM_018043056.1 | 138 |
| R:5′-GGAAACAATACAGGACCTCATA-3’ | |||
| ACSL1 | F:5′-GGGAGGCGAGCAGAAAGA-3’ | XM_018041881.1 | 142 |
| R:5′-AACAGACGGTGGAGGGTG-3’ | |||
| ACSS1 | F:5′-ACCTGAGAAGGGATGTGG-3’ | XM_018057282.1 | 104 |
| R:5′-AGAAGACTCAACGCAAACA-3’ | |||
| FAS | F:5′-TTGCCTAAAGTCAGTGTCG-3’ | NM_001285629.1 | 169 |
| R:5′-GCAGGTCAACCGCATAAC-3’ | |||
| ACACA | F:5′-GCTTTCTTCACCGAGGCT-3’ | XM_018064168.1 | 173 |
| R:5′-CGGAGGGTATCGCATTCA-3’ | |||
| ACSS2 | F:5′-CCTCCCGTTCTGCTTTCC-3’ | XM_005688483.3 | 132 |
| R:5′-AGCCGTGCCTGGTGGTGTTG-3’ | |||
| SCD | F:5′-CCCCAGTGCCCATCCATTT-3’ | NM_001285619.1 | 168 |
| R:5′-TCCCTTTCTCCTCGGCTTCTC-3’ |