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. Author manuscript; available in PMC: 2018 Sep 29.
Published in final edited form as: Circ Res. 2017 Aug 8;121(8):923–929. doi: 10.1161/CIRCRESAHA.117.310996

Figure 1. Comparison of SpCas9/gRNAs and SaCas9/gRNAs for deletion of exon 23 of the mouse Dmd gene in C2C12 cells.

Figure 1

(A) Diagram showing the genomic locus of mouse Dmd around exon 23. The gRNA targeting sites and sequences for both SpCas9 and SaCas9 were labeled. The mutant exon 23 is highlighted in red. (B) PCR analysis of genomic DNA extracted from C2C12 cells treated without or with SpCas9/gRNAs or SaCas9/gRNAs. (C) Quantitative RT-PCR analysis of the dystrophin expression in C2C12 cells treated without or with SpCas9/gRNAs or SaCas9/gRNAs. (D) DNA sequencing analysis of RT-PCR product of SaCas9-gRNA transduced C2C12 cells. *P<0.05 and **P<0.01. All data are representative of a minimum of three experiments.