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. Author manuscript; available in PMC: 2018 Sep 29.
Published in final edited form as: Circ Res. 2017 Aug 8;121(8):923–929. doi: 10.1161/CIRCRESAHA.117.310996

Figure 3. Restoration of dystrophin in the cardiomyocytes of mdx/Utr+/− mice.

Figure 3

(A) Measurement of dystrophin-positive cardiomyocytes (% of total caveolin-3-positive cardiomyocytes; mean ± SD) in mdx/Utr+/− heart cryosections treated with or without 1 × 1012 vg AAV-SaCas9/gRNAs (N=4 per group). (B) RT-PCR analysis of heart tissues from mdx/Utr+/− mice treated with (N=5) or without (N=3) 1 × 1012 vg AAV-SaCas9/gRNA. Genome editing is expected to produce a smaller (~500 bp) dystrophin band. (C) Immunoblotting of heart lysates from WT, mdx/Utr+/− and mdx/Utr+/− with AAV-SaCas9/gRNAs using anti-dystrophin and anti-GAPDH antibodies. (D) Quantitative analysis of dystrophin expression by immunoblotting of heart lysates from wild-type (WT), mdx/Utr+/− and AAV treated mdx/Utr+/− (N=3 per group). # p < 0.01.