Figure 1. TLR2 and TLR6 play a role in platelet activation induced by oxPC_CD36.

(A, F) Murine platelets of indicated genotypes (WT, MyD88^−/−, TLR2^−/−, and TLR6^−/−) were isolated by gel filtration and incubated with representative member of oxPC_CD36 (KODA-PC, 20 μM), ADP (10 μM), or thrombin (0.05 U/ml), and integrin α_IIbβ₃ activation was determined by FACS analysis using PE-conjugated integrin α_IIbβ₃ (JON/A) antibody. (A) Left panels present FACS analysis data as histograms, (A) right panel and (F) present data as mean ± SD of 3 independent experiments. (B–E, G–H) Human platelets were isolated by gel filtration and pre-incubated with either (B, C) 2 μM MyD88 blocking peptide (BP), or control peptide (CP) or (D, E) 4 μg/ml TLR2 neutralizing antibody, or (G, H) 4 μg/ml TLR6 neutralizing antibody or non-immune isotype matched control IgG followed by stimulation with oxPC_CD36 (KODA-PC, 20 μM). P-selectin expression was assessed by FACS analysis. FACS analysis data are presented as histograms and as mean ± SD of 3 independent experiments. * p<0.05, *** p<0.001, and NS p>0.05 vs control.