Figure 3.

Fine mapping of sscqsc5.1 and sucqsc5.1 on genome scaffold CM3.5.1_scaffold00022 on chromosome 5 using the PS × SC5-1 subNILs. Genomic regions containing PS alleles are indicated in white, SC in black, heterozygous in light gray, and regions containing a recombination break point, in dark gray. (A) QTL location was narrowed to an 82.4 kbp interval between sca00022_3650228 and CMPSNP437_3732580 in 16 F₃ subNILs as indicated by significant differences in SSC (^*) in the table (top right) according to Dunnett's test (p < 0.05) compared to PS. Means ± SD. (B) Two recombinant lines and an additional 6 SNPs further delimited a 56.1 kbp interval between sca00022_3672395 and sca00022_3728529 as indicated by significant differences in SSC and sucrose in CDM14 (middle table left) and VAL14 (middle table right) in eight F₄ subNILs, compared to PS. (C) Predicted candidate genes (green) and transposons (blue) contained in the QTL interval according to physical position on the genome scaffold.