Table 1.

Comparisons of three OG sequencing methods.

	METHOD A Sequencing OG by formation of a deletion signature	METHOD B Sequencing OG by conversion to an unnatural base pair	METHOD C Sequencing OG by OG-Seq
Suitable Sequencing Platforms	Sanger	Sanger & NGSa	NGS
DNA Contexts	Targeted sequencing of plasmids or genomic loci	Targeted sequencing of plasmids or genomic loci & the genomic scalea	Genomic scale
Resolution of sequenced OG	Single nucleotide	Single nucleotide	~150 bps
Ability to detect more than one OG per strand	Yes	No & Yesa	No
Custom chemical synthesis required	No	Yes	No

^aThe ability to sequence OG by conversion to an unnatural DNA base pair on an NGS platform, the genomic scale, and more than one OG per strand analyzed is achievable as soon as the d5SICS, dNaM, and dMMO2 unnatural DNA bases can be sequenced on a commercial NGS platform. The concept of sequencing d5SICS and dNaM with the MspA protein nanopore has been demonstrated (Craig et al., 2015).