Table 1. Synthesis of 11cROL (pmol/mg protein).

Retinoid isomerase activities of WT and the indicated mutant RPE65s were determined by measuring synthesis of 11-cis retinol (11cROL) at 30°C or 37°C. Numbers indicate 11cROL content (pmol ± SD, n = 3) in 1 mg of cellular protein (middle columns) or ratio of the isomerase activities at 30°C to those at 37°C (right column). NA, no activity.

Mutation	Activity at 30°C	Activity at 37°C	Ration 30/37°C
L22P	22 ± 3	4 ± 1	5.5
T101I	12 ± 2	2 ± 1	6.0
L408P	26 ± 3	5 ± 1	5.2
H180R	NA	NA	–
H313R	NA	NA	–
Y239D	1.3 ± 0.3	1.3 ± 0.5	1.0
C330Y	1.8 ± 0.3	1.6 ± 0.3	1.1
E417Q	1.2 ± 0.4	1.1 ± 0.4	1.1
WT	124 ± 10	138 ± 10	0.9