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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Virology. 2017 Jun 16;511:309–319. doi: 10.1016/j.virol.2017.06.005

Fig. 2. Viral loads in peritoneal leukocytes (PLs), brain and kidneys of tadpoles at different time following infection with WT-, Δ52L-, Δ64R- or Δ18-FV3.

Fig. 2

Outbred pre-metamorphic tadpoles were infected by i.p. injection of 1 × 104 PFU of each virus type and FV3 genome copy numbers in PLs, brains and kidneys at 2, 6 and 12 dpi were determined by absolute qPCR using primers specific for FV3 vDNA Pol II. (A–C) Viral loads for Δ52L- Δ64R- and WT-FV3. (D–F) Viral loads for Δ18K- and the same WT-FV3 control as in A. Results are means ± SE of genome copy number/100µ of genomic DNA of 6 to 10 animals per group and are representative from two different experiments. **; P <0.001 and *; P <0.05 significant differences between WT and KO-FV3 using one-way ANOVA test and Tukey’s post hoc test.