Fig. 5.

Infection with HIV-1 induces up-regulation of lnc173 expression in a manner dependent on dose and time. qRT-PCR with RNA from different cell lines after infection with VSV-G-pseudotyped HIV-1 NL4-3 Δenv/EGFP. Cells were lysed and RNA was harvested 24 h after infection, unless stated otherwise. (A) Infection of HEK 293T cells with serial 4-fold dilutions. Results are the average of 4 independent experiments. (B) Infection of THP-1 cells with serial 4-fold dilutions. Results are the average of 4 independent experiments. (C) Infection of Jurkat cells with undiluted HIV-1. Results are the average of 3 independent experiments. (D) Infection of HEK 293T cells in a time course with harvest after 12, 24, 36 and 48 h. Expression values are relative to time-matched control samples. Results are the average of 3 independent experiments. (E) Exposure of HEK 293T cells to heat-inactivated HIV-1. Virus was heat-inactivated for 30 min at 65 °C prior to infection where indicated. Results are the average of 3 independent experiments. Error bars indicate standard deviation. RPLP0 was used as reference gene.