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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Virology. 2017 Aug 23;511:74–81. doi: 10.1016/j.virol.2017.08.025

Fig. 3. Lysine Free APOBEC3B shows wild-type levels of HIV-1 restriction.

Fig. 3

(A) Infectivity of Vif-deficient HIV-1IIIB produced in 293T cells expressing increasing amounts of the indicated expression constructs (0 ng, 25 ng, 50 ng, or 100 ng of A3-coding plasmid balanced with empty vector up to 100 ng to ensure equal DNA amounts). Each histogram bar is the average +/- SD of technical triplicate infections (p-values obtained by Welch two-tailed t-test). Immunoblots of the indicated cellular and viral proteins in whole cell extracts and virus-like particles with tubulin and p24 as loading controls, respectively.

(B) Infectivity of Vif-deficient HIV-1LAI produced in 293T cells expressing a single amount of the indicated expression constructs (25 ng). Each histogram bar is the average +/- SD of technical triplicate infections (p-values obtained by Welch two-tailed t-test). Immunoblots of the indicated cellular and viral proteins in whole cell extracts and virus-like particles with tubulin and p24 as loading controls, respectively.