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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2017 Aug 3;1861(11 Pt A):2883–2890. doi: 10.1016/j.bbagen.2017.08.005

Figure 2. Separation of sulfated 4-androstene-3,17-dione and related steroids by thin layer chromatography (TLC).

Figure 2

The reaction mixtures following the sulfotransferase assay were analyzed by TLC procedures as described in Materials and Methods. The signals were detected by autoradiography using the Fluoro Image Analyzer (FLA-3000). Compounds analyzed were: lane 1, DMSO (as a control); lane 2, sulfated 4-androstene-3,17-dione; lane 3, sulfated 1,4-androstadiene-3,17-dione; lane 4, sulfated DHEA.