FIGURE 2.
Pseudomonas aeruginosa stringent response strains in a cutaneous mouse infection model. CD-1 mice were subcutaneously infected with either PAO1 or LESB58, their corresponding stringent response mutants ΔrelA and ΔrelA/ΔspoT, as well as the ΔrelA/ΔspoT complemented strains. A high bacterial density (5 × 107 CFU) was used. Lesion sizes (Box and whiskers plot) and CFU counts (with geometric mean) were determined 3 days post-infection. (A) PAO1 dermonecrosis measurements. (B) PAO1 CFU counts/abscess. (C) LESB58 dermonecrosis measurements. (D) LESB58 CFU counts/abscess. (A–D) All experiments were done at least three times independently with 2–4 mice/group. Statistical analysis was performed using one-way ANOVA, Kruskal–Wallis test with Dunn’s correction. The asterisk indicates a significant difference to the wild-type (p < 0.05).