Table 1.
Bacterial strains used in this study.
| Strain | Relevant characteristics or genotypea | Reference or source |
|---|---|---|
| Escherichia coli | ||
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 (rK- mK+) supE44 relA1 lac,[F′ proAB lacIq ZΔM15Tn10 (Tcr)] | Stratagene |
| ST18 | pro thi hsdR+ Tpr Smr; chromosome::RP4-2 Tc::Mu-Km::Tn7/aaapir ΔhemA | Thoma and Schobert, 2009 |
| Sm17λpir | Tpr Smr recA, thi, pro, hsdR-M+RP4: 2-Tc:Mu: Km, aaapir phage lysogen | Simon et al., 1983 |
| Pseudomonas aeruginosa | ||
| PAO1 | Laboratory wild-type strain | Hancock and Carey, 1979 |
| PAO1.ΔrelA | relA deletion mutant | This study |
| PAO1.ΔrelA/ΔspoT | ΔrelA/ΔspoT double deletion mutant | This study |
| PAO1.ΔrelA/ΔspoT (complement relA) | ΔrelA/ΔspoT deletion mutant chromosomally complemented with the relA gene including its promoter region | This study |
| PAO1.ΔrelA/ΔspoT (complement spoT) | ΔrelA/ΔspoT deletion mutant chromosomally complemented with the spoT gene including the rpoZ-spoT promoter region | This study |
| PAO1.(16S-lux) | Chromosomal insertion of the 16S promoter region fused to the luxABCDEF reporter genes | This study |
| PAO1.(relA-lux) | Chromosomal insertion of the relA promoter region fused to the luxABCDEF reporter genes | This study |
| PAO1.(spoT-lux) | Chromosomal insertion of the rpoZ-spoT promoter region fused to the luxABCDEF reporter genes | This study |
| LESB58 | Liverpool Epidemic Strain isolate | Cheng et al., 1996 |
| LESB58.ΔrelA | relA deletion mutant | This study |
| LESB58.ΔrelA/ΔspoT | ΔrelA/ΔspoT double deletion mutant | This study |
| LESB58.ΔrelA/ΔspoT (complement relA) | ΔrelA/ΔspoT deletion mutant chromosomally complemented with the relA geneincluding its promoter region | This study |
| LESB58.ΔrelA/ΔspoT (complement spoT) | ΔrelA/ΔspoT deletion mutant chromosomally complemented with the spoT gene including its promoter region | This study |
aAntibiotic resistance: Gmr, gentamicin; Tcr, tetracycline; Tpr, trimethoprim; Smr, streptomycin; Km, kanamycin.