Table 2.
Relative fold-changes of P. aeruginosa PAO1 ΔrelA/ΔspoT and LESB58 ΔrelA/ΔspoT mRNA expression compared to their respective wild-type levels of expression.
| Gene | Locus (PAO1/LESB58) | Virulence factor/description | Fold change in ΔrelA/ΔspoT |
|
|---|---|---|---|---|
| PAO1 | LESB58 | |||
| aprA | PA1249/PALES_40631 | Alkaline protease, Type I secretion | -11.2 | -4.8 |
| lasB | PA3724/PALES_12581 | Elastase type II secretion system substrate | -10.6 | 1.8 |
| pa1L | PA2570/PALES_27241 | Galactose binding lectin A | -10.2 | -1.7 |
| phzB1 | PA4211/PALES_07161 | Phenanzine biosynthesis type II secretion | -4.3 | 1.2 |
| rhlB | PA3478/PALES_15341 | Rhamnolipid rhamnosyltransferase chain B | -3.3 | -1.5 |
| plcB | PA0026/PALES_00251 | Phospholipase C, Type II secretion | -3.0 | -1.1 |
| lipA | PA3996/PALES_22021 | Lipase A type II secretion system substrate | -1.4 | -2.7 |
| exoS | PA3841/PALES_11331 | Exoenzyme S type III secretion substrate | -1.4 | n.d. |
| pscC | PA1716/PALES_36131 | Type III secretion outer membrane protein | 1.3 | 1.2 |
| pchB | PA4230/PALES_06971 | Pyochelin siderophore biosynthesis | 1.4 | -1.5 |
| toxA | PA1148/PALES_41711 | Exotoxin A type II secretion system substrate | 1.8 | -2.4 |
| pscI | PA1722/PALES_36071 | Type III secretion export protein | 2.1 | n.d. |
| pvdE | PA2397/PALES_28991 | Pyoverdine siderophore ABC transporter | 2.1 | -2.0 |
| exsD | PA1714/PALES_36151 | Type III secretion system negative regulator | 3.8 | 1.6 |
| pscF | PA1719/PALES_36101 | Type III secretion needle | 4.3 | 1.3 |
| exsA | PA1713/PALES_36161 | Type III secretion system transcriptional activator | 4.6 | 1.2 |
Strains were grown on KB agar plates supplemented with 10% blood for 2 (PAO1) and 3 (LESB58) days, respectively. Transcript abundance was determined by qRT-PCR and represent the means of at least three biological replicates. n.d., not determined.