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. 2017 Sep 27;8:1226. doi: 10.3389/fimmu.2017.01226

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Copyright © 2017 Azeredo, Machado, Afonso, Fierro-Castro, Reyes-López, Tort, Gesto, Conde-Sieira, Míguez, Soengas, Kreuz, Wuertz, Peres, Oliva-Teles and Costas.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantitative expression of immune-related genes in the head-kidney (A,B) and blood (C,D) in juvenile European seabass fed CTRL (), MET (), or TRP () for 14 days (0 h) and sampled at 4 or 24 h post Hank’s balanced salt solution (HBSS) or Phdp i.p. injection (mean ± SD, n = 6). Metalloproteinase 9 [mmp9, (A)], complement factor 3 [c3, (B)] interleukin-1β [il1β, (C)], and dicentracin [dctrn, (D)]. x and y denote significant differences attributed to sampling time. a and b stand for significant differences between dietary treatments. (A,B) stand for significant differences between stimuli. One-way ANOVA (before i.p. injection) and Multifactorial ANOVA (after i.p. injection); Tukey post hoc test; p ≤ 0.05.

Inline graphic — Quantitative expression of immune-related genes in the head-kidney (A,B) and blood (C,D) in juvenile European seabass fed CTRL (), MET (), or TRP () for 14 days (0 h) and sampled at 4 or 24 h post Hank’s balanced salt solution (HBSS) or Phdp i.p. injection (mean ± SD, n = 6). Metalloproteinase 9 [mmp9, (A)], complement factor 3 [c3, (B)] interleukin-1β [il1β, (C)], and dicentracin [dctrn, (D)]. x and y denote significant differences attributed to sampling time. a and b stand for significant differences between dietary treatments. (A,B) stand for significant differences between stimuli. One-way ANOVA (before i.p. injection) and Multifactorial ANOVA (after i.p. injection); Tukey post hoc test; p ≤ 0.05.