Table 1.
Ingredients and proximate composition of experimental diets as percentage of dry matter (% DM).
| Experimental diets |
|||
|---|---|---|---|
| CTRL | TRP | MET | |
| Ingredients (% DM) | |||
| Fish meala | 34.1 | 33.5 | 33.2 |
| Soybean mealb | 15.0 | 15.0 | 15.0 |
| Corn glutenc | 10.0 | 10.0 | 10.0 |
| Wheat glutend | 5.0 | 5.0 | 5.0 |
| Wheat meale | 16.7 | 16.6 | 16.2 |
| Fish oil | 13.9 | 13.4 | 14.0 |
| Vitamin premixf | 1.0 | 1.0 | 1.0 |
| Choline chloride (50%) | 0.5 | 0.5 | 0.5 |
| Mineral premixg | 1.0 | 1.0 | 1.0 |
| Binderh | 1.0 | 1.0 | 1.0 |
| Agar | 1.0 | 1.0 | 1.0 |
| Dibasic calcium phosphate | 0.84 | 0.91 | 0.96 |
| l-Methioninei | – | – | 1.16 |
| l-Tryptophani | – | 0.52 | – |
| Proximate analyses (% dry weight) | |||
| DM (%) | 95.2 | 94.9 | 94.3 |
| Crude protein | 44.9 | 45.2 | 45.0 |
| Crude lipid | 15.5 | 16.5 | 16.9 |
| Ash | 10.5 | 10.4 | 10.5 |
aPesquera Centinela, Steam Dried LT, Chile (CP: 71.4%; CL 9.3%). Sorgal, S.A. Ovar, Portugal.
bSoybean meal (CP: 54.9%; CL:2.1%), Sorgal, S.A. Ovar, Portugal.
cCorn gluten (CP: 72.2%; CL: 2.0%), Sorgal, S.A. Ovar, Portugal.
dWheat gluten (CP: 84.4%; CL: 2.1%), Sorgal, S.A. Ovar, Portugal.
eWheat meal (CP: 13.9%; CL: 1.8%), Sorgal, S.A. Ovar, Portugal.
fVitamins (mg kg−1 diet): retinol, 18,000 (IU kg−1 diet); calciferol, 2,000 (IU kg−1 diet); alpha tocopherol, 35; menadion sodium bis., 10; thiamin, 15; riboflavin, 25; Ca pantothenate, 50; nicotinic acid, 200; pyridoxine, 5; folic acid, 10; cyanocobalamin, 0.02; biotin, 1.5; ascorbyl monophosphate, 50; inositol, 400.
gMinerals (mg kg−1 diet): cobalt sulfate, 1.91; copper sulfate, 19.6; iron sulfate, 200; sodium fluoride, 2.21; potassium iodide, 0.78; magnesium oxide, 830; manganese oxide, 26; sodium selenite, 0.66; zinc oxide, 37.5; dicalcium phosphate, 8.02 (g kg−1 diet); potassium chloride, 1.15 (g kg−1 diet); sodium chloride, 0.4 (g kg−1 diet).
hAquacube. Agil, UK.
iFeed grade amino acids, Sorgal, SA. Ovar, Portugal.