Table 1.
Range, accuracy, intermediate precision, and specificity of relative binding assay. LLOQ, lower limit of quantitation; LQC, low‐quality control; MQC, middle‐quality control; HQC, high‐quality control; ULOQ, upper limit of quantitation
| Fcγ receptor | Range (μm) | Test sample | Nominal value (IgG; (μm) | Accuracy (%) | Intermediate precision (%) | Specificity: % binding of IgA |
|---|---|---|---|---|---|---|
| FcγRIIIa | 0.104–3.33 | LLOQ | 0.1 | 96.6 | 7.75 | |
| LQC | 0.2 | 97.6 | 5.59 | 0.5 | ||
| MQC | 0.9 | 106.0 | 5.33 | 1.8 | ||
| HQC | 2.7 | 84.4 | 8.08 | |||
| ULOQ | 3.1 | 93.8 | 5.13 | |||
| FcγRIIIb | 0.832–26.67 | LLOQ | 0.9 | 110.6 | 4.48 | |
| LQC | 3.1 | 87.1 | 2.70 | 9.9 | ||
| MQC | 6.7 | 91.5 | 5.79 | 13.2 | ||
| HQC | 20.0 | 104.4 | 7.12 | |||
| ULOQ | 22.2 | 104.3 | 11.99 | |||
| FcγRIIa | 0.104–3.33 | LLOQ | 0.1 | 111.4 | 7.99 | |
| LQC | 0.2 | 90.6 | 4.09 | 1.4 | ||
| MQC | 0.9 | 92.0 | 7.50 | 3.3 | ||
| HQC | 2.7 | 101.5 | 12.11 | |||
| ULOQ | 3.1 | 101.4 | 7.33 | |||
| FcγRIIb | 0.832–26.67 | LLOQ | 0.9 | 106.2 | 5.93 | |
| LQC | 3.1 | 90.3 | 3.64 | 14.3 | ||
| MQC | 6.7 | 96.1 | 4.45 | 27.9 | ||
| HQC | 20.0 | 101.5 | 6.81 | |||
| ULOQa | 22.2 | 101.0 | 6.83 |
a
One sample was excluded due to an air bubble in the injection; n = 17. All other results are based on n = 18.