Table 3.
Summarized results of stressed IgG1 samples on Fc receptor binding. n.d., not determined. F‐T, freeze‐thaw cycles; DG, deglycosylation; AF, afucosylation
| Stress condition | Relative binding (%) | Affinity (nm) | Fraction bound (%) | ||||
|---|---|---|---|---|---|---|---|
| FcγRIIIa | FcγRIIIb | FcγRIIa | FcγRIIb | FcγRI | FcRn | FcRn | |
| Reference | 100.0 | 100.0 | 100.0 | 100.0 | 0.56 | 5.7 | 8.3 |
| H2O2_0.1% | 103.6 | 93.1 | 103.9 | 107.4 | 0.93 | n.d. | n.d. |
| H2O2_0.25% | 102.8 | 101.1 | 104.5 | 103.5 | 0.53 | 6.2 | 6.4 |
| H2O2_0.5% | 97.4 | 94.9 | 100.1 | 100.1 | 0.42 | 6.2 | 7.7 |
| pH7.2_48 h | 97.4 | 89.6 | 140.3 | 92.9 | 0.59 | 5.5 | 7.6 |
| pH7.2_72 h | 92.7 | 86.5 | 143.9 | 92.9 | 0.48 | 6.1 | 8.3 |
| pH7.2_96 h | 96.9 | 88.3 | 132.1 | 94.7 | 0.58 | 5.6 | 7.9 |
| pH8.0_48 h | 59.5 | 52.5 | 76.6 | 54.4 | 0.52 | 6.0 | 7.8 |
| pH8.0_72 h | 53.2 | 53.8 | 64.5 | 53.6 | 0.55 | 5.5 | 6.5 |
| pH8.0_96 h | 61.8 | 65.9 | 78.2 | 61.8 | 0.55 | 5.3 | 10.0 |
| 40 °C‐1000 r.p.m._24 h | 96.8 | 106.1 | 92.5 | 101.1 | 0.53 | 5.5 | 7.9 |
| 40 °C‐1000 r.p.m._48 h | 101.8 | 105.9 | 91.8 | 100.1 | 0.45 | 5.0 | 7.3 |
| 40 °C‐1000 r.p.m._72 h | 102.9 | 107.1 | 97.5 | 103.1 | 0.53 | 5.4 | 8.6 |
| 70 °C_15 m | 72.7 | 126.7 | 62.5 | 93.4 | n.d. | 34.2a | n.d. |
| 75 °C_15 m | 388.7 | > 1000 | > 1000 | > 1000 | n.d. | 0.4a | n.d. |
| F‐T 1 | 80.1 | 106.5 | 102.2 | 102.3 | 0.52 | 5.7 | 8.4 |
| F‐T 5 | 103.0 | 104.7 | 92.3 | 97.2 | 0.42 | 5.7 | 8.1 |
| F‐T 10 | 98.7 | 102.0 | 85.9 | 95.9 | 0.48 | 5.2 | 7.4 |
| DG 0 | 106.2 | 104.9 | 99.5 | 105.3 | 0.52 | 4.9 | 8.5 |
| DG 100 | 6.5 | 10.5 | 10.3 | 15.3 | No fit | 6.3 | 4.1 |
| AF 3 | 79.7 | 83.9 | 101.5 | 90.6 | 0.44 | 3.7 | 14.3 |
| AF 8 | 87.7 | 93.0 | 97.6 | 84.2 | 0.43 | 3.7 | 14.1 |
| AF 70 | 158.6 | 186.3 | 103.5 | 110.5 | 0.33 | 3.8 | 15.7 |
a
Nonspecific binding to reference channels was measured; kinetics on FcRn could not be determined.