Table 5.
Summary of Fc tail interactions to monitor for changes in product characteristics
| IgG modification | FcγRIIIa | FcγRIIIb | FcγRIIa | FcγRIIb | FcγRI | FcRn |
|---|---|---|---|---|---|---|
| Deamidation (10–50%) | Reduced relative binding | Reduced relative binding | Reduced relative binding | Reduced relative binding | No impact | No impact |
| DG (100%) | Hardly any binding | Hardly any binding | Hardly any binding | Hardly any binding | Hardly any binding | Slightly faster off‐rate. Lower fraction bound at neutral pH |
| Aberrant fucosylation (3–70%) | Increased binding with lower fucosylation | Increased binding with lower fucosylation | No impact | No impact | No impact | No impact |
| Aggregation (5–75%) | Higher relative binding (> 400%) | Higher relative binding (> 400%) | Higher relative binding (> 400%) | Higher relative binding (> 400%) | Slower off‐rate, increased K D | Slower off‐rate, increased K D, 1 : 1 binding model |
| Ox (< 7% on Met252) | No impact | No impact | No impact | No impact | No impact | No impact |
| Thermal/shake stress | No impact | No impact | No impact | No impact | No impact | No impact |
| F/T | No impact | No impact | No impact | No impact | No impact | No impact |
Results in boldface indicate results that have not been reported in the literature to authors’ knowledge.