Figure 5.

miR‐30d reverses EMT marker gene and protein expression. The level of EMT marker gene in NCI‐H2452 cells was significantly reversed post‐transfection with miR‐30d mimics compared with the NC. (A) Expression levels of CDH1, VIM, and TWIST1 gene were determined by qRT‐PCR and normalized against the endogenous control ACTIN. (B) Expression levels of CDH1, VIM, and TWIST1 proteins were determined by western blot analysis and normalized against the endogenous control ACTIN/H3. (C) The expression of proteins hybridizing bands was detected by western blot analysis. Data were represented as the mean ± SD. *P < 0.05, **P < 0.01. All assays were performed in duplicate.