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The glutamate-stimulated ROS was effectively attenuated by biatractylolide in PC12 and SH-SY5Y cell lines. After the two cell lines were treated with various concentrations of biatractylolide for 30 min before glutamate treatment for 24 hours, the ROS production was detected by ROS kit. (a) The fluorescence image. (b)-(c) Bar chart shows quantitative data. ∗p < 0.05 and ∗∗p < 0.01 versus control group; #p < 0.05 and ##p < 0.05 versus model group.
