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. 2017 Aug 10;11(10):1307–1329. doi: 10.1002/1878-0261.12075

Figure 3.

Figure 3

KLK4 regulates gene expression through PAR1 in prostate‐derived stromal cells. (A) Gene expression was studied by RTqPCR in WPMY1 cells treated for 6, 12 or 18 h with mKLK4, KLK4 (20 nm), AP1 or AP2 (100 μm). PBS treatment was used as reference for each time point. Results are presented as mean ± SD of three biological replicates. (B) Gene expression was investigated by RTqPCR in WPMY1 cells transfected with PAR1‐siRNA or control‐siRNA treated with KLK4 or mKLK4 (20 nm) for 18 h. Expression in WPMY1 cells control‐siRNA treated with mKLK4 was used as reference. Results are presented as mean of relative mRNA expression ± SD of three biological replicates. (C) Gene expression was determined by RTqPCR in WPMY1 cells treated for 6 h with mKLK4, KLK4 (20 nm), AP1 or AP2 (100 μm) in the presence of 0.3 and 0.7 μm of PAR1 inhibitor (SHC79797) or vehicle control (DMSO). Gene expression after mKLK4 treatment was used as reference for each concentration of inhibitor. Results are presented as mean ± SD of three biological replicates. *P < 0.05, **P < 0.01, ***P < 0.001 compared to reference.