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. 2017 Aug 4;9(10):1415–1433. doi: 10.15252/emmm.201607012

Figure EV1. CDK6 knockdown sensitizes OVCAR8 cells to platinum‐induced cell death.

Figure EV1

  1. Table summarizing CDDP and CBDCA IC50 of OVCAR8 cells transduced with ctrl or CDK6‐specific shRNAs. Results are expressed as percentage of viable cells with respect to untreated cells and the resulting IC50 (half maximal inhibitory concentrations) are reported (n = 3 performed in triplicate).
  2. Growth curve of control or CDK6 silenced OVCAR8 cells. Data represent the mean ± SD of two biological replicates (each performed in triplicate). The corresponding cell lysates were analyzed by Western blot for CDK6 expression. Vinculin was used as loading control
  3. Cell cycle distribution of OVCAR8 cells transduced with control or CDK6‐specific shRNAs evaluated by flow cytometry 72 h post‐transduction. The corresponding cell lysates were analyzed by Western blot for CDK6 expression. Tubulin was used as loading control.
  4. Western blot evaluating PARP expression and cleavage and γH2AXS139 and CDK6 expression in OVCAR8 cells transduced with control or CDK6 shRNA and treated with 60 μg/ml of CBDCA for 16 h (P) and the released in platinum‐free medium for the indicated times. V indicates control cells treated with vehicle. Tubulin was used as loading control.
  5. Cell viability of OVCAR8 cells transfected with CDK6 WT or empty vector and treated with increasing doses of CDDP for 16 h. Results are expressed as percentage of viable cells with respect to untreated cells. Data represent the mean ± SD of three biological replicates (two‐sided, unpaired t‐test). The corresponding cell lysates were analyzed by Western blot for CDK6 expression. GRB2 was used as loading control.
Data information: In each panel, significant differences are evidenced by asterisks (*P < 0.05, ***P < 0.001) and the exact P‐values of (E) are reported in Appendix Table S4.Source data are available online for this figure.