Figure 5. Intact positioning of GnRH neurons in KlbKO mice.

• A
  Representative brain sections from wild‐type and KlbKO adult females showing GnRH neuron cell bodies and fibers at the level of the ovlt in the POA (upper panel) and at the level of the median eminence in the mediobasal hypothalamus (ME, lower panel) level. Scale bars, 200 μm.
• B
  Analysis of GnRH neuron distribution along the rostro‐caudal axis from the level of the medial septum/diagonal band of broca (MS/DBB, −1,080 μm) through the organum vasculosum of the lamina terminalis (ovlt, 0 μm), to the level of the median eminence (ME, +2,160 μm). WT: n = 7; KO: n = 4.
• C
  Quantification of total hypothalamic GnRH neurons in adult KlbKO females and WT littermates (WT: n = 8; KO: n = 4). Differences in GnRH cell number between groups were assessed using unpaired t‐test.
• D, E
  Gene expression profiles in immature (Gn11) and mature (GT1‐7) immortalized GnRH neurons and dissected tissues at embryonic day E13.5 (nose and brain) and different postnatal ages from postnatal day 0 (P0) to adulthood (n ≥ 3 per group). Differences between groups were assessed using one‐way ANOVA followed by Fisher's LSD test.

Data information: Values shown are mean ± SEM; different letters (abcde) indicate significant differences between groups (P < 0.05).