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. 2017 Jul 28;9(10):1379–1397. doi: 10.15252/emmm.201607376

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© 2017 The Authors. Published under the terms of the CC BY 4.0 license

This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Figure EV5 — A
Representative photomicrographs of the preoptic region showing GnRH neurons (GFP, green) and laminin immunoreactivity surrounding the blood vessels of the OVLT (arrows, red) labeled by fluorescent rFGF21 (5 nmol/animal, white staining). 3V, third ventricle; OVLT, organum vasculosum laminae terminalis.

B
Representative photomicrographs showing laminin immunoreactivity surrounding the blood vessels of the ME (arrows, red) labeled by fluorescent rFGF21 (5 nmol/animal, white staining) in the tuberal region of the hypothalamus. Scale bar 40 μm (for A and B).