Figure EV5. Effect of Rab7a or FIP200 depletion on LLPD and LDH sequestration.

1. U2OS cells transfected with control siRNA or siRNA against Rab7a or FIP200 were subjected to a LLPD assay. The quantification of the degradation rate for long‐lived proteins is presented as mean ± s.e.m. of at least three independent experiments.
2. U2OS cells transfected with control siRNA or siRNA against Rab7a or FIP200 were subjected to a LDH sequestration assay. In the graph, the net sequestered LDH per hour (%/h) is represented as mean ± s.e.m. of at least three independent experiments.

Data information: Statistical significance was evaluated in (A and B) using paired Student's t‐test. *P < 0.05; **P < 0.01.