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. 2017 Aug 30;9(10):1356–1365. doi: 10.15252/emmm.201707672

Figure 3. The TREM2 variant p.H157Y impairs phagocytosis.

Figure 3

Phagocytic activity was measured using an E. coli pHrodo uptake assay. Cells were incubated with E. coli pHrodo particles for either 1 or 2 h (gray and black bars, respectively), and populations of phagocytically active cells were determined using flow cytometry. Cytochalasin D was used as a control. Data are shown as mean ± SD from four independent experiments (n EV(1 h) = 11, n WT(1 h) = 11, n EV(2 h) = 12, n WT(2 h) = 12) and three and two independent experiments for 1‐ and 2‐h treatments, respectively (n p.H157Y(1 h) = 8, n p.H157Y(2 h) = 6). One‐way ANOVA (with Tukey's post hoc test) was used for statistical analysis; EV versus WT (1 h): ***P = 0.0005; EV versus p.H157Y (1 h): P = 0.413; WT versus p.H157Y (1 h): *P = 0.0141; EV versus WT (2 h): ***P = 0.000014; EV versus p.H157Y (2 h): P = 0.071; WT versus p.H157Y (2 h): **P = 0.0031.