Figure EV2. The MYS‐1 HAT complex regulates the expression of DAF‐16 but not other well‐known genes involved in lifespan regulation.

1. Total RNA was extracted from the control or MYS‐1‐overexpressing worms, and the expression levels of daf‐16 mRNA or sod‐3 mRNA were determined by qRT–PCR. Error bars represent the SD derived from two independent experiments. **P < 0.01, ***P < 0.001, one‐way ANOVA followed by Tukey's test. The value of the control worms was set to 1.
2. N2 worms were treated with the indicated RNAi, and total RNA was extracted at day 2 adulthood. The level of each mRNA was determined by qRT–PCR. The value of the control RNAi‐treated worms was set to 1. The bars represent the relative mRNA level from three independent experiments. Error bars, SD.
3. Total RNA was extracted from the indicated RNAi‐treated N2 worms at day 2 adulthood, and the expression level of daf‐16 mRNA was determined by qRT–PCR. Error bars represent the SD derived from three independent experiments. The value of the control RNAi‐treated worms was set to 1 (left). Worm extracts from the indicated RNAi‐treated N2 worms or DAF‐16::GFP worms at day 2 adulthood were subjected to immunoblot analysis using an anti‐DAF‐16 antibody, respectively (right). Representative images of two independent experiments are shown.