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. 2017 Sep 8;18(10):1837–1853. doi: 10.15252/embr.201744147

Figure 6. lncRNA‐PAGBC requires miR‐133b and miR‐511 to promote growth and metastasis and activate the AKT/mTOR pathway in GBC cells.

Figure 6

  1. Western blotting analysis of p‐AKT, t‐AKT, p‐mTOR and t‐mTOR in the indicated GBC cell clones.
  2. Transwell migration and invasion assays of SGC‐996 or EH‐GB1 cells that were transfected with the indicated plasmids. Scale bars represent 100 μm.
  3. The proliferation of SGC‐996 or EH‐GB1 cells was measured using CCK‐8 assays. The data are shown as the means ± SD of triplicate samples.
  4. Western blotting analysis of p‐AKT, t‐AKT, p‐mTOR and t‐mTOR in EH‐GB1 cells transiently transfected with the indicated plasmids.
  5. Transwell migration and invasion assays of NOZ and EH‐GB1 cells stably transfected with lentiviruses encoding the indicated transcripts and treated with the indicated microRNA inhibitors or mimics. Scale bars represent 100 μm.
  6. The proliferation of NOZ and EH‐GB1 cells was measured using CCK‐8 assays. The data are shown as the means ± SD of triplicate samples.
  7. Western blotting analysis of p‐AKT, t‐AKT, p‐mTOR and t‐mTOR in EH‐GB1 cells transfected with lentiviruses encoding the indicated transcripts and treated with the indicated microRNA mimics.
Data information: *P < 0.05, **P < 0.01, ***P < 0.001 (Student's t‐test). Source data are available online for this figure.