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. 2017 Sep 8;18(10):1837–1853. doi: 10.15252/embr.201744147

Figure 7. PABPC1 interacts with lncRNA‐PAGBC and increases the stability of lncRNA‐PAGBC .

Figure 7

  1. RNA pull‐down assays were performed in NOZ cells. The two bands highlighted by the arrow were submitted for mass spectrometry analysis.
  2. Upper panel: Western blot analysis following RNA pull‐down assays performed using NOZ cellular extracts. An antibody against the β‐actin protein was used as the negative control. Lowe panel: RIP experiments were performed on extracts from NOZ cells using an antibody against PABPC1.
  3. PABPC1 protein expression levels in GBC cells were determined by Western blot analysis.
  4. qRT–PCR was performed to measure the expression of lncRNA‐PAGBC in GBC cells. The data are shown as the means ± SD.
  5. After dividing the 77 GBC tissue samples into two groups according to PABPC1 expression, the expression levels of lncRNA‐PAGBC were compared. The data are shown as the means ± SD.
  6. NOZ cells were transfected with a siRNA against PABPC1 for 48 h and then treated with actinomycin D (10 μmol/l) to block RNA synthesis. lncRNA‐PAGBC levels were measured via qRT–PCR and normalized to the level of GAPDH. All values at time 0 h were normalized to 1.
Data information: ***P < 0.001, **P < 0.01 (Student's t‐test). Source data are available online for this figure.