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. 2017 Sep 27;8:1197. doi: 10.3389/fimmu.2017.01197

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Copyright © 2017 Darling, Sherwood and Hayhurst.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Exploring the impact of coexpressing the anti-Marburgvirus (MARV) single-domain antibody (sdAb) monomer (M1) or dimer (M2), or anti-Ebolavirus (EBOV) monomer (E1) or dimer (E2) on nucleoprotein (NP) incorporation into VLP. (A) Sliver stained SDS-PAGE analysis of crude VLP preparations following coexpression of MARV NP and/or VP40 with the various sdAb monomers and dimers. (B) Coomassie stained SDS-PAGE analysis of cell lysates stemming from the VLP production in panel (A). (C) Sliver stained SDS-PAGE analysis of crude VLP preparations following coexpression of EBOV NP and/or VP40 with the various sdAb monomers and dimers. (D) Coomassie stained SDS-PAGE analysis of cell lysates stemming from the VLP production in panel (C).