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. 2017 Sep 27;8:1197. doi: 10.3389/fimmu.2017.01197

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Copyright © 2017 Darling, Sherwood and Hayhurst.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Production and distribution of single-domain antibody (sdAb) dimers within the Vero E6 based stable cell lines. (A) Western blot of stable cell lines expressing the anti-Marburgvirus (MARV) dimer (Vero-M2) or anti-Ebolavirus (EBOV) dimer (Vero-E2) and probing for antibody via the C9 tag or for β-actin. (B) Staining of parent (Vero-wt) and stable cell lines Vero-M2 and Vero-E2 for the distribution of antibody via the C9 tag. (C) Transient expression of MARV HA-NP within the parental and transgenic cell lines demonstrates the ability of the anti-MARV dimer but not the anti-EBOV dimer to colocalize with nucleoprotein (NP) puncta. (D) Transient expression of EBOV HA-NP within the parental and transgenic cell lines demonstrates the ability of the anti-EBOV dimer but not the anti-MARV dimer to colocalize with NP puncta.