Figure 9.

Impact of Xintrabodies on viral replication. Marburgvirus (MARV) (A) and Ebolavirus (EBOV) (B) were titrated on wild-type Vero cells (wt) or the stable lines expressing the anti-MARV dimer (M2) or anti-EBOV dimer (E2) and plaque formation under semi-solid overlay allowed to proceed for 10 days. The experiment was performed twice for MARV and three times for EBOV and error bars represent the SD. Diameters of approximately 150 plaques from the titrations were measured using ImageJ measure analysis tool and plotted for MARV (C) and EBOV (D). An unpaired, one-tail t-test analysis revealed a p-value of <0.0001 (****) while ns indicates no significant difference. Bars indicate SD and the mean. (E) MARV and EBOV were used to infect wild-type or cognate single-domain antibody dimer stable cell lines in a growth kinetics format with liquid supernatant collections at the times indicated. Plaque titrations of the supernatants were performed on wild-type cells and results are graphed as a percentage of titers resulting from the wild-type time-course. The experiment was performed three times for MARV and twice for EBOV. An unpaired, one-tail t-test was conducted using Excel, * indicating a p-value <0.05 and ** indicating a p-value <0.005.