FIGURE 1.

OFP1 interacts with DLT and itself in yeast and plant cells. (A) Yeast two-hybrid analyses of the indicated proteins on minimal synthetic defined media containing -Leu/-Trp or -Leu/-Trp/-His/-Ade dropout supplements. (B) Schematic diagram of OFP1 protein with NES, NLS, and OVATE domain indicated. Alignment of the putative NES with the common NES sequence was shown. (C) Subcellular co-localization of OFP1-GFP with RFP or DLT-RFP. BF, bright field. Scale bars = 10 μm. (D,E) BiFC analysis of the interactions between the indicated proteins. Nucleus was magnified to show the punctate structure. Chl, chloroplast fluorescence; BF, bright field. Scale bars = 10 μm.