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Single-Round PCR amplification of DNA extracted from fecal of Entamoeba complex positive samples. Lane 1, 50 bp ladder (Cat NO. PR901633); Lane 2, negative control; Lane 3, positive control: a mixture of standard DNA of E. histolytica (166 bp), E. dispar (752 bp), and E. moshkovskii (580 bp); lane 4–5 two cyst positive samples; line 4, 752 bp amplification showed E. dispar-positive infection; line 5, 580 and 752 bp amplified products showed co-infection of E. moshkovskii and E. dispar.
