FIGURE 4.

Effect of EGTA, BAPTA/AM, TFP, W-7, W-5, LaCl₃ or nifedipine on adventitious rooting under osmotic stress. The primary roots were removed of 6-day-old seedlings. And then explants were incubated for 6 days with distilled water (control) or 0.05% (w/v) PEG 6000, PEG + 10 μM SNP, PEG + 200 μM CaCl₂, PEG + 10 μM SNP + 200 μM CaCl₂, PEG + 10 μM SNP + 100 μM EGTA, PEG + 10 μM SNP + 30 μM BAPTA/AM, PEG + 10 μM SNP + 100 μM TFP, PEG + 10 μM SNP + 80 μM W-7, PEG + 10 μM SNP + 80 μM W-5, PEG + 10 μM SNP + 500 μM LaCl₃ or PEG + 10 μM SNP + 150 μM nifedipine. Adventitious root numbers (A) and root length (B) were expressed as mean ± SE (n = 3, 10 explants were used per replicate). Bars with different lower case letters were significantly different by Duncan’s multiple range test (p < 0.05).