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. 2017 Sep 27;8:1684. doi: 10.3389/fpls.2017.01684

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Copyright © 2017 Niu, Yu, Liao, Yu, Zhang and Dawuda.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of NO and CaCl₂ on TBARS (A), H₂O₂ (B) and O₂^-(C) of cucumber leaf under osmotic stress. Explants were incubated with distilled water (control) or 0.05% (w/v) PEG 6000, PEG + 10 μM SNP, PEG + 200 μM CaCl₂, PEG + 10 μM SNP + 100 μM EGTA, PEG + 10 μM SNP + 30 μM BAPTA/AM, PEG + 10 μM SNP + 500 μM LaCl₃, PEG + 10 μM SNP + 150 μM nifedipine, PEG + 10 μM SNP + 100 μM TFP or PEG + 10 μM SNP + 80 μM W-7. The content of TBARS, H₂O₂ and O₂^- in cucumber were determined after 48 h of treatment, respectively. Bars with different lower case letters were significantly different by Duncan’s multiple range test (p < 0.05).