FIGURE 5.

Depth and reproducibility of relative quantification of the miRNA target proteins of Table 1 in TDA/DDA and DDA control method experiments. (A) Average numbers of heavy/light peptide pairs detected and quantified for each individual targeted protein, and for all targeted proteins combined, per technical replicate in each batch of LC-MS/MS experiments conducted on sample 1 (left) and sample 2 (right). Statistically significant differences between TDA/DDA and DDA control method experiments, as determined using 2-tailed t-tests, are labeled ^∗p < 0.05 or ^∗∗p < 0.01. (B) Numbers of targeted proteins quantified (from ≥ 2 heavy/light peptide pairs) across all three sets of technical replicates in TDA/DDA and DDA control method experiments conducted on sample 1 (left) and sample 2 (right).