Figure 4.

Schematic representation illustrating the phosphorylation sites on p66ShcA. There are three tyrosine phosphorylation sites and a threonine phosphorylation residue in the CH1 domain. There is one serine phosphorylation site on (Serine 138) the PTB domain as well as two serine phosphorylation sites in the amino terminal CH2 domain. p66ShcA has a unique cytochrome c binding region (CB). Ser36, Ser59, Ser138 and Thr386 are involved in the oxidative stress response, whereas S154 has a role in the directional movement of pancreatic cells. The phosphorylation of tyrosine residues in the CH1 domain are involved in MAPK activation (Adapted from Rajendran et al. 2010).