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. 2017 Oct 2;3:17066. doi: 10.1038/cddiscovery.2017.66

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The effect of decrease in Atg7 protein levels on the expression of differentiation markers. (a) Cells were transfected with siRNA against Atg7 (one sequence) and respective non-targeting siRNA. AICAR (0.5 mM), ATRA (1 μM), metformin (15 mM) or PMA (50 nM) were added 24 h after transfection. Total cell lysates were isolated 48 h after addition of agents and analyzed by western blot. The expression of differentiation markers was analyzed by flow cytometry 48 or 72 h after addition of agents. Western blot analyses and data obtained by flow cytometry are shown for each of the two independent experiments (Exp 1–2). (b) Cells were transfected with siRNA against Atg7 (four sequences) and respective non-targeting siRNA. AICAR (0.5 mM), ATRA (1 μM), metformin (15 mM) or PMA (50 nM) were added 24 h after transfection. Total cell lysates were isolated 3 or 48 h after addition of agents and analyzed by western blot. The expression of differentiation markers was analyzed by flow cytometry 48 or 72 h after addition of agents. Western blot analyses and data obtained by flow cytometry are shown for each of the two independent experiments (Exp 1–2).