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. 2017 Sep 29;5:e3789. doi: 10.7717/peerj.3789

Figure 4. The effect of 9-cis-RA on the expression of T adhaerens NRs.

Figure 4

A representative experiment of the expression of T. adhaerens NRs in animals exposed to various concentrations of 9-cis-RA expressed as a ratio of obtained values compared to the control using ddPCR. One and 3 nM 9-cis-RA upregulate RXR and ERR, but downregulate COUP-TF. The expression of T. adhaerens HNF4 is not affected by 9-cis-RA. The effect of the exposure to 9-cis-RA is stronger in the case of 1 nM 9-cis-RA compared to 3 nM 9-cis-RA. The exposure to 30 nM, as well as 50 nM concentrations of 9-cis-RA reverse the effect of 9-cis-RA on the expression of RXR and COUP-TF, but do not influence the expression of ERR. The level of expression of HNF4 is not changed by exposure of T. adhaerens to various concentrations of 9-cis-RA. The data suggest that a network sensitive to nanomolar concentrations of 9-cis-RA at the expressional level is formed by RXR, COUP-TF and ERR. All four NRs have conserved P-box (regions responsible for binding to response elements (RE) in gene promoters) and are likely to bind overlapping REs and to form a functional network.