Figure 2.

Retinal cell types differ in their spatial distributions. a–d: Example fields of horizontal cells (a), cholinergic amacrine cells (b), Type 2 cone bipolar cells (c) and Type 4 cone bipolar cells (d), from B6/J retinas. Each field has been masked of all non-somal labeling, in order to emphasize the spatial patterning of each cellular population. Notice that none of the mosaics forms a precisely hexagonal lattice, yet those for the horizontal cells and cholinergic amacrine cells appear more regular in their patterning. In both of these mosaics, cells are occasionally adjacent to one another; this occurs with higher frequency, however, in the two bipolar cell mosaics. Indeed, bipolar cells of the same type occasionally appear to be compressed aside one another (e.g. yellow arrowheads in c and d), yielding an intercellular spacing that is less than the average diameter derived from measurements of their somal areas. Occasionally, bipolar cells are found isolated from nearby neighbors (e.g. orange arrows in c and d). Calibration bar = 50 μm.