Table 2.
Effects of PcdhgC3 knockout on gene expression in mouse brain microvascular endothelial cell line cerebEND.
| Gene symbol | PcdhgC3KO relative to wild-type cells set as 1 |
|---|---|
| Tight junctions: | |
| Cldn3 | 0.7 ± 0.1 (*) |
| Cldn5 | 4.4 ± 1.71 (*) |
| Cdh5 | 2.2 ± 0.77 |
| Ocln | 0.2 ± 0.07 (*) |
| Tjp1 | 2.6 ± 0.79 (*) |
| Mesenchymal markers: | |
| Fn-1 | 1.0 ± 0.38 |
| Snail | 1.0 ± 0.18 |
| Vim | 1.4 ± 0.27 |
| mTOR pathway: | |
| mTOR | 0.8 ± 0.1 (*) |
| Sqstm-1 | 0.4 ± 0.19 (*) |
| Wnt pathway: | |
| Axin-1 | 0.7 ± 0.08 (*) |
| Fzd-1 | 1.4 ± 0.2 (*) |
| Gsk3b | 0.8 ± 0.12 (*) |
| Lrp-5 | 0.6 ± 0.12 (*) |
| Pard3 | 0.6 ± 0.18 (*) |
Cdh5: cadherin 5 (VE-cadherin); Cldn: claudin; Fn-1: fibronectin-1; Fzd-1: Wnt/fizzled; Gsk3b: glycogen synthase kinase 3 beta; Lrp-5: LDL receptor-related protein 5; mTOR: mechanistic target of rapamycin; Ocln: occludin; Pard3: Par-3 family cell polarity regulator; Snail: snail family zinc finger 1; Sqstm-1: sequestosome 1; Tjp1: tight junction protein 1 (ZO-1); Vim: vimentin.
Values represent at least four different experiments and data are presented as mean ± SD. Statistical significance was evaluated using t-test and p values lower than 0.05 were considered as statistically significant (*).