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. Author manuscript; available in PMC: 2018 Mar 20.
Published in final edited form as: Nature. 2017 Sep 20;549(7673):548–552. doi: 10.1038/nature24023

Extended data Figure 8. CYREN inhibits cNHEJ preferentially at breaks with overhangs by preventing processing.

Extended data Figure 8

a, Percentage of cells using HR to repair Cas9-induced breaks. Detail of 4 wt and 4 KO clones used in Fig. 5b. b, Deletion profiles of repair of Cas9-induced breaks. Detail profiles of 4 wt and 4 KO clones used in Fig. 5c. Blue line in blunt ends: break site. Blue area: overhang region created by the pair of sgRNAs. c. Average percentage of mClover+ cells in 4 wt clones, 4 KO clones, 4 KO clones complemented with wt CYREN and 4 KO clones complemented with mutant CYREN (RPW-AAA), in 3 independent experiments, normalized to wt. Whiskers: Tuckey. *P<0.05. Unpaired T test. In each experiment, 100,000 cells per sample were analysed. d, Deletion profiles of repair of Cas9 breaks. Average percentage of deletion in wt, KO clones, and KO clones complemented with wt CYREN or mutant CYREN (RPW-AAA). Error bars, s.e.m.