Extended data Figure 2. Chromatid-type fusions induced by CYREN depletion in IMR90 fibroblasts.
a, Western blot showing TRF2 depletion. For gel source data, see Supplementary Figure 1. b, qRT-PCR measurement of CYREN isoforms expression after siRNA knock-down. Normalized to ACTB qRT-PCR. CYREN-1, PCR primers target mRNA transcript variant 1. CYREN-2, PCR primers target mRNA transcript variants 2, 3, 4 and 5. CYREN-3, PCR primers target mRNA transcript variant 7. c, Representative images of partial metaphase spreads of functional (shControl) and deprotected (shTRF2) telomeres after CYREN depletion. d, Mean percentage of fused chromosome ends per metaphase. Error bars, s.e.m. **P<0.01. One-way ANOVA, Sidak’s multiple comparison test. n: number of metaphases analysed.